@g-man: Sorry to confuse you..this was a very work-specific post. In simple language, I finally succeeded in an experiment that I have been working on for last few months.
@ g-man: I don't know your academic background, but I can try to explain since you are interested.
I was trying to find if a certain protein is involved in a certain biochemical process. So I had to detect that protein in my samples. It's a long process (check the wiki link if you want to know more). Finally you get to see black blobs on a X-ray film (a pic is there in the wiki link) that tells you that particular protein is there.
Unlike course work labs, no one can give a printed directions in actual research. So we have to keep trying different things until we get a conclusive result. So that's what I got on Friday..and it showed that my hypothesis was correct. So I was feeling on top of the world :)
@ Samby: he he ok. I am not good at writing for readers. This is still like a diary to me most of the times. But I will try to keep readers in mind since some people are actually reading my random thoughts :)
well, i'm an engineer :) but i think i got the gist of what you're trying to say - i've got a few friends doing biotechnology and biochemical engineering. it looks something like a dna test actually :) don't they use gel electrophoresis there too? n yay for you!
@ Pavi: You are so sweet :) It was very technical I know. Was just too happy to finally get the result I was trying to get for quite some time.
@ g-man: Yes you are absolutely right. It is very much like DNA gel electrophoresis...just some chemicals used are different. However electrophoresis is only part of this process. Electrophoresis only separates all components present. It doesn't tell which is which. So for that, we then do 'immunoblots' that specifically picks up only our desired component from all the other stuff (kind of like using a magnet to pick up only iron from mixture of similar looking metals). So ultimately if you get those 'black blobs' you know it was definitely there in the mixture.
Wow, I'm almost giving a class here :p It is very nice to see an engineer interested in biology though :)
well i'm interested in certain aspects of biology. totally not into studying the entire human body. experiments like this, and genes and stuff, definitely! you make a pretty good teacher :)
13 comments:
huh?
:-)
Posts like these are what remind me when I tend to forget what I love about science
congrats!
@g-man: Sorry to confuse you..this was a very work-specific post.
In simple language, I finally succeeded in an experiment that I have been working on for last few months.
@tgfi: Days like these remind me the same :)
ummm...okay. would i even understand?
yeah well i dnt know much about plants..so please be more explanatory next time..:P
i showed stomata in my 12th board practicals and thats it...:-)....no slicing leaves again
@ g-man: I don't know your academic background, but I can try to explain since you are interested.
I was trying to find if a certain protein is involved in a certain biochemical process. So I had to detect that protein in my samples. It's a long process (check the wiki link if you want to know more). Finally you get to see black blobs on a X-ray film (a pic is there in the wiki link) that tells you that particular protein is there.
Unlike course work labs, no one can give a printed directions in actual research. So we have to keep trying different things until we get a conclusive result. So that's what I got on Friday..and it showed that my hypothesis was correct. So I was feeling on top of the world :)
Hope my explanation makes some sense to you :)
@ Samby: he he ok. I am not good at writing for readers. This is still like a diary to me most of the times. But I will try to keep readers in mind since some people are actually reading my random thoughts :)
Are u talking latin??? Anywez..glad ur happy...guess thats all that matters eventually :)
well, i'm an engineer :) but i think i got the gist of what you're trying to say - i've got a few friends doing biotechnology and biochemical engineering. it looks something like a dna test actually :) don't they use gel electrophoresis there too? n yay for you!
@ Pavi: You are so sweet :)
It was very technical I know. Was just too happy to finally get the result I was trying to get for quite some time.
@ g-man: Yes you are absolutely right. It is very much like DNA gel electrophoresis...just some chemicals used are different. However electrophoresis is only part of this process. Electrophoresis only separates all components present. It doesn't tell which is which. So for that, we then do 'immunoblots' that specifically picks up only our desired component from all the other stuff (kind of like using a magnet to pick up only iron from mixture of similar looking metals). So ultimately if you get those 'black blobs' you know it was definitely there in the mixture.
Wow, I'm almost giving a class here :p It is very nice to see an engineer interested in biology though :)
well i'm interested in certain aspects of biology. totally not into studying the entire human body. experiments like this, and genes and stuff, definitely! you make a pretty good teacher :)
i dint understand a,b, c of it....do u do ur PhD in bio or stuff like that ?
@g-man: Thanks :)
@ashu: yes, I am in biology.
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